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algorithms imagej schneider
Algorithms Imagej Schneider, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 3995 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/algorithms+imagej+imagej/CFX+Maestro+Software/pm41722047-185-241-252
Average 98 stars, based on 3995 article reviews
Algorithms Imagej Schneider, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 3995 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/algorithms+imagej+imagej/CFX+Maestro+Software/pm41722047-185-241-252
Average 98 stars, based on 3995 article reviews
algorithms imagej schneider - by Bioz Stars,
2026-09
98/100 stars
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Knockdown:Article Title: Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and algorithms ImageJ ImageJ https://imagej.nih.gov/ij/ Image Lab Bio-rad N/A Illustrator Adobe N/A Photoshop Adobe N/A Other Dulbecco’s modified eagle medium (DMEM) Welgene Cat# LM001-05 Fetal bovine serum (FBS) Gibco Cat# 26140079 70 mm cell strainer Falcon Cat# 352350 AriaMx Real-time PCR system Agilent N/A QuantStudio 1 Real-time PCR system Thermo Scientific N/A .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and Reverse Transcription:Article Title: Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and algorithms ImageJ ImageJ https://imagej.nih.gov/ij/ Image Lab Bio-rad N/A Illustrator Adobe N/A Photoshop Adobe N/A Other Dulbecco’s modified eagle medium (DMEM) Welgene Cat# LM001-05 Fetal bovine serum (FBS) Gibco Cat# 26140079 70 mm cell strainer Falcon Cat# 352350 AriaMx Real-time PCR system Agilent N/A QuantStudio 1 Real-time PCR system Thermo Scientific N/A .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and Mutagenesis:Article Title: Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and algorithms ImageJ ImageJ https://imagej.nih.gov/ij/ Image Lab Bio-rad N/A Illustrator Adobe N/A Photoshop Adobe N/A Other Dulbecco’s modified eagle medium (DMEM) Welgene Cat# LM001-05 Fetal bovine serum (FBS) Gibco Cat# 26140079 70 mm cell strainer Falcon Cat# 352350 AriaMx Real-time PCR system Agilent N/A QuantStudio 1 Real-time PCR system Thermo Scientific N/A .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and Software:Article Title: Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and algorithms ImageJ ImageJ https://imagej.nih.gov/ij/ Image Lab Bio-rad N/A Illustrator Adobe N/A Photoshop Adobe N/A Other Dulbecco’s modified eagle medium (DMEM) Welgene Cat# LM001-05 Fetal bovine serum (FBS) Gibco Cat# 26140079 70 mm cell strainer Falcon Cat# 352350 AriaMx Real-time PCR system Agilent N/A QuantStudio 1 Real-time PCR system Thermo Scientific N/A .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and Article Title: 5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation Article Snippet: .. 340 STAR METHODS 341 KEY RESOURCES TABLE 342 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- Serotonin Abcam Cat# Ab315150; RRID: AB_3697404 Anti-Transglutaminase 2 Cell Signaling Technology Cat# 3557; RRID: AB_2202883 Anti-Ki67 Rabbit pAb Servicebio Cat# GB111499 RRID: AB_2927572 Anti- β-Actin Proteintech Cat# 81115-1-RR; RRID: AB_2923704 Goat Anti-Rabbit IgG (H+L) Earthox Cat# E031620 RRID: AB_3065690 Goat Anti-Mouse IgG (H + L) HRP MultiSciences Cat# GAM0072 RRID: AB_3065690 Goat Anti-Rabbit IgG (H + L) HRP MultiSciences Cat# GAR007 RRID: AB_3073716 Biological samples Preterm infant blood samples Children’s Hospital of Nanjing Medical University Ethics No: 202110094-1 Chemicals, peptides, and recombinant proteins Jo urn al Pr e-p roo f 5-HT MCE Cat# HY-B1473A 5-PT MCE Cat# HY-156562 LB-533401 MCE Cat# HY-15849 ZED-1227 TargetMol Cat# T17289 DMEM/F12 Gibco Cat# 11320033 FBS Gibco Cat# 10099141C penicillin‒streptomycin Gibco Cat# 15070063 RIPA Beyotime Cat# P0013B Protease inhibitor cocktail and phosphatase inhibitor Beyotime Cat# P1046 SBE-β-CD MCE Cat# HY-17031 DMSO Beyotime Cat# ST038 PEG300 Beyotime Cat# Y268724 Tween 80 Beyotime Cat# ST2789 biotin-azide Thermo Fisher Cat# B10184 magnetic streptavidin beads MCE Cat# HY-K0208 Critical commercial assays Click-iT Cell Reaction Buffer Kit Thermo Fisher Cat# C33372 TGM2 Activity Assay Kit Novus Biologicals Cat# NBP1-37008 5-HT ELISA Kit Elabscience Cat# E-EL-0033 Cell Counting Kit-8 Beyotime Cat# C0038 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062M TUNEL Cell Apoptosis Detection Kit Servicebio G1507-50T Experimental models: Cell lines MLE-12 ATCC BH-C465 Experimental models: Organisms/strains Lung of C57BL/6 wild-type mice Animal Center of Nanjing Medical University N/A Software and Modification:Article Title: Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and algorithms ImageJ ImageJ https://imagej.nih.gov/ij/ Image Lab Bio-rad N/A Illustrator Adobe N/A Photoshop Adobe N/A Other Dulbecco’s modified eagle medium (DMEM) Welgene Cat# LM001-05 Fetal bovine serum (FBS) Gibco Cat# 26140079 70 mm cell strainer Falcon Cat# 352350 AriaMx Real-time PCR system Agilent N/A QuantStudio 1 Real-time PCR system Thermo Scientific N/A .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and Real-time Polymerase Chain Reaction:Article Title: Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and algorithms ImageJ ImageJ https://imagej.nih.gov/ij/ Image Lab Bio-rad N/A Illustrator Adobe N/A Photoshop Adobe N/A Other Dulbecco’s modified eagle medium (DMEM) Welgene Cat# LM001-05 Fetal bovine serum (FBS) Gibco Cat# 26140079 70 mm cell strainer Falcon Cat# 352350 AriaMx Real-time PCR system Agilent N/A QuantStudio 1 Real-time PCR system Thermo Scientific N/A .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines SW1353 ATCC Cat# HTB-94; RRID: CVCL_0543 CHON-001 ATCC Cat# CRL-2846; RRID: CVCL_C462 Experimental models: Organisms/strains Mouse: C57BL/6J DBL N/A Oligonucleotides siRNA for knockdown, see Table S1 Bioneer N/A Primers for RT-qPCR, see Table S2 This paper N/A Primers for strand-specific reverse transcription, see Table S3 This paper N/A Primers for strand-specific RT-qPCR, see Table S4 This paper N/A Sequences for PKR mutagenesis, see Table S5 This paper N/A Software and Recombinant:Article Title: 5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation Article Snippet: .. 340 STAR METHODS 341 KEY RESOURCES TABLE 342 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- Serotonin Abcam Cat# Ab315150; RRID: AB_3697404 Anti-Transglutaminase 2 Cell Signaling Technology Cat# 3557; RRID: AB_2202883 Anti-Ki67 Rabbit pAb Servicebio Cat# GB111499 RRID: AB_2927572 Anti- β-Actin Proteintech Cat# 81115-1-RR; RRID: AB_2923704 Goat Anti-Rabbit IgG (H+L) Earthox Cat# E031620 RRID: AB_3065690 Goat Anti-Mouse IgG (H + L) HRP MultiSciences Cat# GAM0072 RRID: AB_3065690 Goat Anti-Rabbit IgG (H + L) HRP MultiSciences Cat# GAR007 RRID: AB_3073716 Biological samples Preterm infant blood samples Children’s Hospital of Nanjing Medical University Ethics No: 202110094-1 Chemicals, peptides, and recombinant proteins Jo urn al Pr e-p roo f 5-HT MCE Cat# HY-B1473A 5-PT MCE Cat# HY-156562 LB-533401 MCE Cat# HY-15849 ZED-1227 TargetMol Cat# T17289 DMEM/F12 Gibco Cat# 11320033 FBS Gibco Cat# 10099141C penicillin‒streptomycin Gibco Cat# 15070063 RIPA Beyotime Cat# P0013B Protease inhibitor cocktail and phosphatase inhibitor Beyotime Cat# P1046 SBE-β-CD MCE Cat# HY-17031 DMSO Beyotime Cat# ST038 PEG300 Beyotime Cat# Y268724 Tween 80 Beyotime Cat# ST2789 biotin-azide Thermo Fisher Cat# B10184 magnetic streptavidin beads MCE Cat# HY-K0208 Critical commercial assays Click-iT Cell Reaction Buffer Kit Thermo Fisher Cat# C33372 TGM2 Activity Assay Kit Novus Biologicals Cat# NBP1-37008 5-HT ELISA Kit Elabscience Cat# E-EL-0033 Cell Counting Kit-8 Beyotime Cat# C0038 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062M TUNEL Cell Apoptosis Detection Kit Servicebio G1507-50T Experimental models: Cell lines MLE-12 ATCC BH-C465 Experimental models: Organisms/strains Lung of C57BL/6 wild-type mice Animal Center of Nanjing Medical University N/A Software and Protease Inhibitor:Article Title: 5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation Article Snippet: .. 340 STAR METHODS 341 KEY RESOURCES TABLE 342 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- Serotonin Abcam Cat# Ab315150; RRID: AB_3697404 Anti-Transglutaminase 2 Cell Signaling Technology Cat# 3557; RRID: AB_2202883 Anti-Ki67 Rabbit pAb Servicebio Cat# GB111499 RRID: AB_2927572 Anti- β-Actin Proteintech Cat# 81115-1-RR; RRID: AB_2923704 Goat Anti-Rabbit IgG (H+L) Earthox Cat# E031620 RRID: AB_3065690 Goat Anti-Mouse IgG (H + L) HRP MultiSciences Cat# GAM0072 RRID: AB_3065690 Goat Anti-Rabbit IgG (H + L) HRP MultiSciences Cat# GAR007 RRID: AB_3073716 Biological samples Preterm infant blood samples Children’s Hospital of Nanjing Medical University Ethics No: 202110094-1 Chemicals, peptides, and recombinant proteins Jo urn al Pr e-p roo f 5-HT MCE Cat# HY-B1473A 5-PT MCE Cat# HY-156562 LB-533401 MCE Cat# HY-15849 ZED-1227 TargetMol Cat# T17289 DMEM/F12 Gibco Cat# 11320033 FBS Gibco Cat# 10099141C penicillin‒streptomycin Gibco Cat# 15070063 RIPA Beyotime Cat# P0013B Protease inhibitor cocktail and phosphatase inhibitor Beyotime Cat# P1046 SBE-β-CD MCE Cat# HY-17031 DMSO Beyotime Cat# ST038 PEG300 Beyotime Cat# Y268724 Tween 80 Beyotime Cat# ST2789 biotin-azide Thermo Fisher Cat# B10184 magnetic streptavidin beads MCE Cat# HY-K0208 Critical commercial assays Click-iT Cell Reaction Buffer Kit Thermo Fisher Cat# C33372 TGM2 Activity Assay Kit Novus Biologicals Cat# NBP1-37008 5-HT ELISA Kit Elabscience Cat# E-EL-0033 Cell Counting Kit-8 Beyotime Cat# C0038 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062M TUNEL Cell Apoptosis Detection Kit Servicebio G1507-50T Experimental models: Cell lines MLE-12 ATCC BH-C465 Experimental models: Organisms/strains Lung of C57BL/6 wild-type mice Animal Center of Nanjing Medical University N/A Software and Activity Assay:Article Title: 5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation Article Snippet: .. 340 STAR METHODS 341 KEY RESOURCES TABLE 342 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- Serotonin Abcam Cat# Ab315150; RRID: AB_3697404 Anti-Transglutaminase 2 Cell Signaling Technology Cat# 3557; RRID: AB_2202883 Anti-Ki67 Rabbit pAb Servicebio Cat# GB111499 RRID: AB_2927572 Anti- β-Actin Proteintech Cat# 81115-1-RR; RRID: AB_2923704 Goat Anti-Rabbit IgG (H+L) Earthox Cat# E031620 RRID: AB_3065690 Goat Anti-Mouse IgG (H + L) HRP MultiSciences Cat# GAM0072 RRID: AB_3065690 Goat Anti-Rabbit IgG (H + L) HRP MultiSciences Cat# GAR007 RRID: AB_3073716 Biological samples Preterm infant blood samples Children’s Hospital of Nanjing Medical University Ethics No: 202110094-1 Chemicals, peptides, and recombinant proteins Jo urn al Pr e-p roo f 5-HT MCE Cat# HY-B1473A 5-PT MCE Cat# HY-156562 LB-533401 MCE Cat# HY-15849 ZED-1227 TargetMol Cat# T17289 DMEM/F12 Gibco Cat# 11320033 FBS Gibco Cat# 10099141C penicillin‒streptomycin Gibco Cat# 15070063 RIPA Beyotime Cat# P0013B Protease inhibitor cocktail and phosphatase inhibitor Beyotime Cat# P1046 SBE-β-CD MCE Cat# HY-17031 DMSO Beyotime Cat# ST038 PEG300 Beyotime Cat# Y268724 Tween 80 Beyotime Cat# ST2789 biotin-azide Thermo Fisher Cat# B10184 magnetic streptavidin beads MCE Cat# HY-K0208 Critical commercial assays Click-iT Cell Reaction Buffer Kit Thermo Fisher Cat# C33372 TGM2 Activity Assay Kit Novus Biologicals Cat# NBP1-37008 5-HT ELISA Kit Elabscience Cat# E-EL-0033 Cell Counting Kit-8 Beyotime Cat# C0038 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062M TUNEL Cell Apoptosis Detection Kit Servicebio G1507-50T Experimental models: Cell lines MLE-12 ATCC BH-C465 Experimental models: Organisms/strains Lung of C57BL/6 wild-type mice Animal Center of Nanjing Medical University N/A Software and Enzyme-linked Immunosorbent Assay:Article Title: 5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation Article Snippet: .. 340 STAR METHODS 341 KEY RESOURCES TABLE 342 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- Serotonin Abcam Cat# Ab315150; RRID: AB_3697404 Anti-Transglutaminase 2 Cell Signaling Technology Cat# 3557; RRID: AB_2202883 Anti-Ki67 Rabbit pAb Servicebio Cat# GB111499 RRID: AB_2927572 Anti- β-Actin Proteintech Cat# 81115-1-RR; RRID: AB_2923704 Goat Anti-Rabbit IgG (H+L) Earthox Cat# E031620 RRID: AB_3065690 Goat Anti-Mouse IgG (H + L) HRP MultiSciences Cat# GAM0072 RRID: AB_3065690 Goat Anti-Rabbit IgG (H + L) HRP MultiSciences Cat# GAR007 RRID: AB_3073716 Biological samples Preterm infant blood samples Children’s Hospital of Nanjing Medical University Ethics No: 202110094-1 Chemicals, peptides, and recombinant proteins Jo urn al Pr e-p roo f 5-HT MCE Cat# HY-B1473A 5-PT MCE Cat# HY-156562 LB-533401 MCE Cat# HY-15849 ZED-1227 TargetMol Cat# T17289 DMEM/F12 Gibco Cat# 11320033 FBS Gibco Cat# 10099141C penicillin‒streptomycin Gibco Cat# 15070063 RIPA Beyotime Cat# P0013B Protease inhibitor cocktail and phosphatase inhibitor Beyotime Cat# P1046 SBE-β-CD MCE Cat# HY-17031 DMSO Beyotime Cat# ST038 PEG300 Beyotime Cat# Y268724 Tween 80 Beyotime Cat# ST2789 biotin-azide Thermo Fisher Cat# B10184 magnetic streptavidin beads MCE Cat# HY-K0208 Critical commercial assays Click-iT Cell Reaction Buffer Kit Thermo Fisher Cat# C33372 TGM2 Activity Assay Kit Novus Biologicals Cat# NBP1-37008 5-HT ELISA Kit Elabscience Cat# E-EL-0033 Cell Counting Kit-8 Beyotime Cat# C0038 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062M TUNEL Cell Apoptosis Detection Kit Servicebio G1507-50T Experimental models: Cell lines MLE-12 ATCC BH-C465 Experimental models: Organisms/strains Lung of C57BL/6 wild-type mice Animal Center of Nanjing Medical University N/A Software and CCK-8 Assay:Article Title: 5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation Article Snippet: .. 340 STAR METHODS 341 KEY RESOURCES TABLE 342 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- Serotonin Abcam Cat# Ab315150; RRID: AB_3697404 Anti-Transglutaminase 2 Cell Signaling Technology Cat# 3557; RRID: AB_2202883 Anti-Ki67 Rabbit pAb Servicebio Cat# GB111499 RRID: AB_2927572 Anti- β-Actin Proteintech Cat# 81115-1-RR; RRID: AB_2923704 Goat Anti-Rabbit IgG (H+L) Earthox Cat# E031620 RRID: AB_3065690 Goat Anti-Mouse IgG (H + L) HRP MultiSciences Cat# GAM0072 RRID: AB_3065690 Goat Anti-Rabbit IgG (H + L) HRP MultiSciences Cat# GAR007 RRID: AB_3073716 Biological samples Preterm infant blood samples Children’s Hospital of Nanjing Medical University Ethics No: 202110094-1 Chemicals, peptides, and recombinant proteins Jo urn al Pr e-p roo f 5-HT MCE Cat# HY-B1473A 5-PT MCE Cat# HY-156562 LB-533401 MCE Cat# HY-15849 ZED-1227 TargetMol Cat# T17289 DMEM/F12 Gibco Cat# 11320033 FBS Gibco Cat# 10099141C penicillin‒streptomycin Gibco Cat# 15070063 RIPA Beyotime Cat# P0013B Protease inhibitor cocktail and phosphatase inhibitor Beyotime Cat# P1046 SBE-β-CD MCE Cat# HY-17031 DMSO Beyotime Cat# ST038 PEG300 Beyotime Cat# Y268724 Tween 80 Beyotime Cat# ST2789 biotin-azide Thermo Fisher Cat# B10184 magnetic streptavidin beads MCE Cat# HY-K0208 Critical commercial assays Click-iT Cell Reaction Buffer Kit Thermo Fisher Cat# C33372 TGM2 Activity Assay Kit Novus Biologicals Cat# NBP1-37008 5-HT ELISA Kit Elabscience Cat# E-EL-0033 Cell Counting Kit-8 Beyotime Cat# C0038 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062M TUNEL Cell Apoptosis Detection Kit Servicebio G1507-50T Experimental models: Cell lines MLE-12 ATCC BH-C465 Experimental models: Organisms/strains Lung of C57BL/6 wild-type mice Animal Center of Nanjing Medical University N/A Software and TUNEL Assay:Article Title: 5-HT promotes bronchopulmonary dysplasia via TGM2-mediated serotonylation Article Snippet: .. 340 STAR METHODS 341 KEY RESOURCES TABLE 342 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- Serotonin Abcam Cat# Ab315150; RRID: AB_3697404 Anti-Transglutaminase 2 Cell Signaling Technology Cat# 3557; RRID: AB_2202883 Anti-Ki67 Rabbit pAb Servicebio Cat# GB111499 RRID: AB_2927572 Anti- β-Actin Proteintech Cat# 81115-1-RR; RRID: AB_2923704 Goat Anti-Rabbit IgG (H+L) Earthox Cat# E031620 RRID: AB_3065690 Goat Anti-Mouse IgG (H + L) HRP MultiSciences Cat# GAM0072 RRID: AB_3065690 Goat Anti-Rabbit IgG (H + L) HRP MultiSciences Cat# GAR007 RRID: AB_3073716 Biological samples Preterm infant blood samples Children’s Hospital of Nanjing Medical University Ethics No: 202110094-1 Chemicals, peptides, and recombinant proteins Jo urn al Pr e-p roo f 5-HT MCE Cat# HY-B1473A 5-PT MCE Cat# HY-156562 LB-533401 MCE Cat# HY-15849 ZED-1227 TargetMol Cat# T17289 DMEM/F12 Gibco Cat# 11320033 FBS Gibco Cat# 10099141C penicillin‒streptomycin Gibco Cat# 15070063 RIPA Beyotime Cat# P0013B Protease inhibitor cocktail and phosphatase inhibitor Beyotime Cat# P1046 SBE-β-CD MCE Cat# HY-17031 DMSO Beyotime Cat# ST038 PEG300 Beyotime Cat# Y268724 Tween 80 Beyotime Cat# ST2789 biotin-azide Thermo Fisher Cat# B10184 magnetic streptavidin beads MCE Cat# HY-K0208 Critical commercial assays Click-iT Cell Reaction Buffer Kit Thermo Fisher Cat# C33372 TGM2 Activity Assay Kit Novus Biologicals Cat# NBP1-37008 5-HT ELISA Kit Elabscience Cat# E-EL-0033 Cell Counting Kit-8 Beyotime Cat# C0038 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat# C1062M TUNEL Cell Apoptosis Detection Kit Servicebio G1507-50T Experimental models: Cell lines MLE-12 ATCC BH-C465 Experimental models: Organisms/strains Lung of C57BL/6 wild-type mice Animal Center of Nanjing Medical University N/A Software and |